Online life science pharma marketplace and platform for products and services.


Current location: Home » Ask » Protein science » Content

N-terminal deletion primers

Pending answer Credit reward to answer the question: 0 -
More information:
Hi all
I need to construct a N-terminal deletion of a protein through PCR. I was told that the best way to do it is to amplify the C-terminal region with a Nde1 and BamH1 recognition site and insert it into a suitable vector.
I have a couple of questions regarding the primer design:
I assume that there has to be an ATG codon just before the C-terminus so that the translation starts there.Am I right??
Also, the BamH1 recognition site is GGATCC....will this additonal insertion in the primer affect its ability to anneal to the parent template?
P.S: I am not a molecular biologist and hence not too familiar with PCR methods.
total 750 hits     Asked by: Anonymouse I want to answer  

[ AskSearch ]  [ ]  [ Tell a friend ]  [ Print ]  [ Close window ]  [ Return to top ]

Search ask
Related questions
Questions pending your answer