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Molecular Biology (Genetics and Biochemistry) Techniques

Molecular Biology overview

Molecular biology concerns with the study of biology at a molecular level. Molecular biology is a multidisciplinary field overlapping with genetics and biochemistry. It studies molecular interactions between DNA, RNA, protein, their biosynthesis and regulations.Genetic engineering, techniques of gene manipulation, is the core component of molecular biology. By definition, gene manipulation concerns the formation of new combinations of heritable material by the insertion of nucleic acid molecules into any vector (virus, bacterial plasmid and other cloning vehicles) so as to allow their incorporation into a host organism in which they do not naturally occur but in which they are capable of continued propagation.

Content

Recombinant DNA cloning
Recombinant DNA Cloning Introduction
b-Galactosidase
DNA cloning Vectors selection
Restriction Endonucleases Enzymes
Type I Restriction Endonucleases
Type II Restriction Endonucleases
Type III Restriction Endonucleases
Restriction Digestion for DNA cloning
Ligase for DNA ligation
DNA Gel Electrophoresis
Pulsed Field Gel Electrophoresis
Capillary Electrophoresis
DNA Molecular Weight Standards (DNA Markers)
DNA gel staining
Ethidium Bromide (EB) Staining Protocol
Ethidium Bromide (EB) Safety Consideration
Ethidium Bromide (EB) Staining Sensitivity
Other DNA Gel Staining Methods
Bacteria Transformation
Competent E.Coli Cells Preparation
Transformation Protocols
X-gal and IPTG Induction
E.Coli Stains Database
Streaking Colonies Protocol
E.Coli Bacterial Stains Storage
Recombinant DNA Libraries Construction
Plaque Hybridization
Colony Hybridization
b-Galactosidase detection
Alpha Complementation
Antibody Screening
BCIP/NBT Color Development
Plasmid Isolation and Purification
Recombinant DNA Cloning Protocols and Online Resources (browse/details)
List of Suppliers
Search DNA Cloning Products Database
References and Suggested Reading

DNA Southern Blot Analysis
Southern Blot Analysis Overview
Genomic DNA Isolation
DNA Quantity and DNA Purity Assays

Nucleic Acids Labelling
Radioactive Labelling of Nucleic Acids
Oligo Probe Labelling
Nonradioactive Labelling of Nucleic Acids
Biotin Probe Labelling Protocol
Southern Blot Autoradiography
Southern Blot Hybridization Protocol - Standard
Southern Blot Protocol - Alkaline Blotting
Southern Blot Protocol - Colony Hybridization
DNA Fragment Purification by Electroelution
Plant Genomic DNA Southern Blot Protocol
References and Suggested Reading

DNA Fingerprinting
Multi-locus Analysis
Single-locus Analysis

DNA Sequencing
DNA Sequencing Protocol
Enzymatic DNA Sequencing Protocol
Chemical DNA Sequencing Protocol
DNA Sequence Analysis
Sequence Alignment Tools
BLAST
FASTA
Sequence Analysis

Telomere Length and Telomerase Assay

RNA Northern Blot Analysis
RNA Northern Blot Overview
RNA Extraction and Purification
Total RNA Isolation
mRNA Isolation
RNA Northern Blot Hybridization - Standard Protocol
RNA Formaldehyde Denaturing Gel
References and Suggested Reading

Polymerase Chain Reaction PCR
RT-PCR
Real-time Quantitative PCR

Epigenetics Technique
Chromatin Immunoprecipitation (CHIP assay)
DNA Methylation Protocols