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Goto: > Log In1M Acetic acid recipe |
| 3ml 98% Acetic Acid (~17 M), add H2O to 50 ml. Reference:
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30% Acrylamide recipe |
| 29g acrylamide 1g of N,N'-methylbisacrylamide Dissolve in 60ml of H2O Adjust the volume to 100ml with H2O. filtration through 0.45 micron pore size filter. Store ambient at 4c. Reference:
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40% Acrylamide (for DNA sequencing) recipe |
| * Dissolve 380 g acrylamide (DNA-sequencing grade) and 20 g N,N’-methylenebisacrylamide. * Add 600 ml distilled water. * Heat to 37 °C to dissolve acrylamide. * Adjust volume to 1 liter with distilled water. * Sterilize by filtration through a 0.45um filter. Reference: Nature protocols
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Alkaline gel loading buffer recipe |
| 1. Add 15 mg bromocresol green, 25 mg xylene cyanol FF, 120 ul 0.5 M EDTA (pH 8.0) in 3 ml 1 M NaOH. 2. Make up volume to 10 ml with distilled water. 3. Store at 4°C. Reference: Nature protocols
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10M Ammonium acetate recipe |
| 770 g ammonium acetate in 800 ml of H2O. Adjust volume to 1 liter with H2O. Sterilize by filtration. Store the solution at 4¡ãC or at room temperature. Do not autoclave. Reference:
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1000x Ampicillin (sodium salt) 100mg/ml recipe |
| 1g ampicillin add H2O to 10ml Sterilize by filtration and aliquote. Store @ -20c. Reference:
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25mg/ml Ampicillin stock solution recipe |
| 1. Dissolve 1 g ampicillin (sodium salt) in 35 ml of distilled water. 2. Make up volume to 40 ml with distilled water. 3. Sterilize by filtration. Reference: Nature protocols
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10% APS recipe |
| 1g ammonium persulfate (APS, MW 228.2) in 10 ml H2O, store fresh in refrigerator. Reference:
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0.5M Ascorbat recipe |
| 4.4g L-Ascorbic acid (MW 176.1) in 50 ml H2O (adjust pH to 6.0 with 1M NaOH) Reference:
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0.5M Ascorbate, pH 6.0 recipe |
| 4.4g L-Ascorbic acid (MW 176.1) in 50 ml H2O (adjust pH to 6.0 with 1M NaOH) Reference:
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0.1M ATP recipe |
| 60mg ATP adjust pH to 7.0 with 0.1M NaOH (pH paper) add ddH2O to final 0.8ml aliquote and freeze @ -20c. Reference:
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1M Bis-Tris, pH 7.0 recipe |
| 20.9g Bis-Tris (MW 209.2) in 100 ml H2O (adjust pH to 7.0 with HCl). Reference:
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10-25x BLOTTO buffer recipe |
| 5% (w/v) nonfat dried milk 0.02% sodium azide in H2O Store at 4¡ãC. Reference:
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2.5M CaCl2 recipe |
| 11 g CaCl2•6H2O in a final volume of 20 ml of H2O. Sterilize by filter. Store at 4¡ãC. Reference:
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1 M CaCl2 (for competent cell) recipe |
| 1. Dissolve 54 g CaCl2·6H2O in 170 ml distilled water. 2. Adjust final volume to 200 ml with distilled water. 3. Sterilize by filtration through a 0.22-micron filter. 4. Store in 1 ml Aliquot at -20 °C. Reference: Nature protocols
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34mg/ml Chloramphenical stock solution recipe |
| 1. Dissolve 1 g chloramphenicol in 25 ml of 100% ethanol. 2. Make up volume to 29.5 ml with 100% ethanol. 3. Aliquot and store at -20 °C for a year. Reference: Nature protocols
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1M Citrate, pH 5.0 recipe |
| 10.5g Citrate.H2O (MW 210) in 50 ml H2O (adjust pH to 5.0 with 10M NaOH). Reference:
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1x DEPC treated H2O recipe |
| add 1ml DEPC (Diethylpyrocarbonate) in 1000ml miniQ H2O, Mix and set at RT for 1 h, autoclave. Reference:
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6x DNA loading dye recipe |
| 25mg bromophenol blue (0.25%), 25mg xylene xyanol (0.25%), 4g sucrose (40%), adjust volume to 10 ml with H2O. Reference:
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1M DTT recipe |
| 770mg Dithiothreitol (DTT, MW 154) in 10 ml H2O. Store at -20c. Reference:
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0.5 M EDTA (pH 8.0) recipe |
| 1. Dissolve 186.1g Na2EDTA·2H2O in 800 ml distilled. 2. pH to 8.0 with NaOH (~20g of NaOH pellets). EDTA will dissolve at pH 8.0. 3. Adjust volume to 1 liter with distilled water. 4. Sterilize by autoclaving and store at room temperature. Reference: Nature protocols
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10 mg/ml Ethidium bromide recipe |
| 1. Dissolve 1g ethidium bromide in 100 ml distilled water. 2. When dissolved, wrap in aluminium foil. Reference: Nature protocols
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6X Gel loading buffer (bromophenol blue and sucrose) recipe |
| 1. Weigh out 25 mg bromophenol blue and 4 g sucrose. 2. Make up volume to 10 ml with distilled water. 3. Store at 4°C. Reference: Nature protocols
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6X Gel loading buffer with Ficoll recipe |
| 1. Dissolve 25 mg bromophenol blue, 25 mg xylene cyanol FF and 1.5 g Ficoll (Type 400; Pharmacia) in 10 ml distilled water. 2. Store at 4°C. Reference: Nature protocols
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6 X Gel loading buffer with glycerol recipe |
| 1. Dissolve 25 mg bromophenol blue and 25 mg xylene cyanol FF 2. Add 3 ml glycerol. 3. Make up volume to 10 ml with distilled water. 4. Store at 4°C. Reference: Nature protocols
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6X Gel loading buffer with sucrose recipe |
| 1. Dissolve 25 mg bromophenol blue, 25 mg xylene cyanol FF and 4 g sucrose in 10 ml distilled water. 2. Store at 4°C. Reference: Nature protocols
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10% Glycerol recipe |
| 1 volume of molecular-biology-grade glycerol in 9 volumes of sterile pure H2O. Sterilize the solution by passing it through a prerinsed 0.22-µm filter. Store in 200ml aliquots at 4¡ãC. Reference:
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1M HCl recipe |
| 4.2ml concentrated HCl (~12M) in 50ml H2O. Reference:
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2.5N HCl recipe |
| 25 ml concentrated HCl (11.6 N) in 91 ml of sterile H2O. Store at room temperature. Reference:
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2 x HEPES-buffered saline recipe |
| 1. Dissolve 1.6 g NaCl, 0.074 g KCl, 0.027 g Na2HPO4·2H2O, 0.2 g dextrose, and 1 g HEPES. 2. Add 90 ml distilled water. 3. Adjust to pH 7.05 with 0.5 M NaOH. 4. Make up volume to 100 ml with distilled water. 5. Sterilize the solution by filtration through a 0.22 micron filter. 6. Store in 5 ml Aliquot at -20 °C. Reference: Nature protocols
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1M IPTG recipe |
| 1.19g Isopropyl-b,D-thiogalactoside (IPTG, MW 238.3) in 5 ml H2O, filter sterilize. Store @ -20c. Reference:
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20% IPTG recipe |
| 2 g IPTG in 8 ml of distilled H2O. Adjust the volume of the solution to 10 ml with H2O and filter sterilize. Dispense and store at -20¡ãC. Reference:
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1 M IPTG recipe |
| 1. Dissolve 2 g IPTG (Isopropylthio-?-D-galactoside) in 8 ml distilled water. 2. Adjust volume to 10 ml with distilled water. 3. Sterilize by filtration through a 0.22 micron filter. 4. Store in 1 ml Aliquot at -20°C. Reference: Nature protocols
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25mg/ml Kanamycin stock solution recipe |
| 1. Dissolve 1 g kanamycin sulfate in 35 ml of distilled water. 2. Make up volume to 40 ml with distilled water. 3. Sterilize by filtration and store at -20 °C. Reference: Nature protocols
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1x LB medium (Luria-Bertani medium) recipe |
| tryptone, 10 g yeast extract, 5 g NaCl, 10 g deionized H2O, to 950 ml Adjust the pH to 7.0 with 5 N NaOH. Adjust the volume to 1 liter with H2O. Sterilize by autoclaving. Reference:
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1M LiCl recipe |
| 2.12g LiCl (MW 42.4) in 50ml H2O, autoclave. Reference:
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Luria Broth recipe |
| 1. Dissolve 25 g Luria broth powder in 750 ml of distilled water. 2. Add 800 ml distilled water. 3. Adjust to pH 7.0 with either HCl or NaOH as appropriate. 4. Make up volume to 1 liter with distilled water. 5. Sterilize by autoclaving. Reference: Nature protocols
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10X M9 salts recipe |
| 1. Dissolve 6.8 g Na2HPO4·7H2O, 3 g KH2PO4, 0.5 g NaCl and 1 g NH4Cl sequentially in 90 ml distilled water. 2. Make up volume to 100 ml with distilled water. 3. Sterilize by autoclaving and store at room temperature. Reference: Nature protocols
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1 M Magnesium acetate recipe |
| 1. Dissolve 214.46 g MgOAc·4H2O in 800ml distilled water. 2. Make up volume to 1 liter with distilled water. 3. Sterilize by filtration. Store at room temperature. Reference: Nature protocols
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1 M MgCl2 recipe |
| 1. Dissolve 203.3g MgCl3·6H2O in 800 ml distilled water. 2. Make up volume to 1 liter with distilled water. 3. Sterilize by autoclaving. Store at room temperature. Reference: Nature protocols
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10X Microtubules stabilizing buffer (10X MTSB) recipe |
| 1. Dissolve 15 G PIPES, 1.9 g EGTA, 1.32 g MgSO4·7H2O and 5 g KOH in 800 ml distilled water. 2. Adjust to pH 7.0 with KOH. 3. Make up volume to 1 liter with distilled water. 4. Sterilize by autoclaving. Reference: Nature protocols
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M9 Minimal Medium recipe |
| 1. Add 1 g NH4Cl and 3 g glucose in 100 ml 10X M9 Salts salution. 2. Add 1 ml 1 M MgSO4 and 10 ml Gibco MEM vitamin solution. 3. Make up volume to 1 liter with distilled water. 4. Check that the pH is 7.0-7.4. 5. Filter sterilize through a 0.22 micron filter. 6. Add 1 ml sterile 100 mM CaCl2 solution. Store at room temperature. Reference: Nature protocols
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10x MOPS electrophoresis buffer recipe |
| 41.8 g MOPS in 700 ml of sterile DEPC-treated H2O. Adjust the pH to 7.0 with 2 N NaOH. Add 20 ml of DEPC-treated 1 M sodium acetate 20 ml of DEPC-treated 0.5 M EDTA (pH 8.0). Adjust the volume to 1 liter with DEPC-treated H2O. Reference:
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5M NaCl recipe |
| 14.6g sodium chloride in 50ml H2O. Reference:
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10M NaOH recipe |
| 40g NaOH in 100ml H2O. Reference:
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1x PBS recipe |
| 8 g NaCl 0.2 g KCl 1.44 g Na2HPO4 0.24 g KH2PO4 in 800 ml of distilled H2O. Adjust the pH to 7.4 with HCl. Add H2O to 1 liter. Sterilize by autoclave. Reference:
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10X PBS recipe |
| 1. Dissolve 2 g KCl, 80 g NaCl, 17.8 g Na2HPO4·2H2O and 2.4 g KH2PO4 in 800 ml distilled water. 2. Make up volume to 1 liter with distilled water. 3. Sterilize by autoclaving. Reference: Nature protocols
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Phenol:chloroform recipe |
| 1. Mix equal amounts of phenol and chloroform. 2. Equilibrate the mixture by extracting several times with 0.1 M Tris (pH 7.6). Reference: Nature protocols
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25:24:1 Phenol:Chloroform:Isoamyl-alchol recipe |
| 25ml Phenol, 24ml Chloroform, 1ml Isoamyl alcohol. Reference:
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10 mM Phenylmethylsulfonyl fluoride (PMSF) recipe |
| 1. Dissolve 17.4 mg PMSF in 10 ml isopropanol. 2. Aliquot and store at -20°C. Reference: Nature protocols
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1 M Potassium acetate (pH 7.5) recipe |
| 1. Dissolve 9.82g potassium acetate in 90 ml distilled water. 2. Adjust to pH 7.5 with 2 M acetic acid. 3. Make up volume to 100 ml with distilled water. 4. Aliquot and store at -20°C. Reference: Nature protocols
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20% SDS recipe |
| 200 g electrophoresis-grade SDS in 900 ml of H2O Heat to 68¡ãC and stir to assist dissolution. Adjust the volume to 1 liter with H2O. Store at room temperature. Do not autoclave. Reference:
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1x SOC recipe |
| tryptone, 20 g yeast extract, 5 g NaCl, 0.5 g add H2O to 950 ml Reference:
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3M sodium acetate (Na-acetate) recipe |
| 408.3 g sodium acetate•3H2O dissolve in 800 ml of H2O Adjust the pH to 5.2 with glacial acetic acid. Adjust to 1 liter with ddH2O Sterilize by autoclaving Reference: Molecular cloning
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2.5M Sodium acetate, pH 5.2 recipe |
| 17g NaAc.3H2O (MW 136) in 35ml H2O, adjust pH to 5.2 with Acetic acid. Adjust volume to 50ml with H2O. Reference:
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20x SSC recipe |
| 175.3 g NaCl 88.2 g sodium citrate in 800 ml of H2O Adjust the pH to 7.0 with a few drops of a 14 N solution of HCl. Adjust the volume to 1 liter with H2O. Dispense into aliquots. Sterilize by autoclaving. Reference:
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20x SSPE recipe |
| 175.3 g NaCl 27.6 g NaH2PO4•H2O 7.4 g EDTA in 800 ml of H2O. Adjust the pH to 7.4 with NaOH. Adjust the volume to 1 liter with H2O. Dispense into aliquots. Sterilize by autoclaving. Reference:
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20mg/ml Streptomycin stock solution recipe |
| 1. Dissolve 1 g streptomycin sulfate in 45 ml of distilled water. 2. Sterilise by filtration and store at -20 °C. Reference: Nature protocols
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1x TAE electrophoresis buffer recipe |
| 242 g of Tris base 57.1 ml of glacial acetic acid 100 ml of 0.5 M EDTA (pH 8.0) in 1000ml H2O. Reference:
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10 X TBE (TRIS/BORATE/EDTA) recipe |
| 1g NaOH MW 40g 108g Tris Base (AKA Trizma Base) MW 121.10g 55g Boric Acid MW 61.83 7.4g EDTA (disodium salt) MW 372.24 Add dry ingredients to 700mls deionized or distilled water in 2 liter flask (water should be stirring for best results) Stir to dissolve. Add deionized or distilled water to bring up total volume to 1 liter. Reference: http://www.sci.wsu.edu/bio/hhp/equipment/buffer_recipe.htm
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1x TBE electrophoresis buffer recipe |
| 54 g of Tris base 27.5 g of boric acid 20 ml of 0.5 M EDTA (pH 8.0) in 1000ml H2O. Reference:
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12.5mg/m Tetracycline stock solution recipe |
| 1. Dissolve 1 g tetracycline in 75 ml of 1:1 vol/vol distilled water:ethanol. 2. Make up volume to 80 ml with 1:1 vol/vol distilled water:ethanol. 3. Sterilize by filtration and wrap aliquots in aluminium foil and stored at -20 °C. Reference: Nature protocols
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100% Trichloroacetic acid (TCA) recipe |
| 1. Dissolve 500 g of TCA in 227 ml distilled water. Reference: Nature protocols
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50 X Tris-acetate for electrophoresis (TAE) recipe |
| 1. Dissolve 242 g Tris base in 800 ml distilled water. 2. Add 57.1 ml glacial acetic acid and 100 ml 0.5 M EDTA (pH 8.0) 3. Make up volume to 1 liter with distilled water. 4. Store at room temperature. Reference: Nature protocols
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5X Tris-borate for electrophoresis (TBE) recipe |
| 1. Dissolve 54 g Tris base and 27.5 g boric acid in 800 ml distilled water. 2. Add 20 ml 0.5 M EDTA (pH 8.0) 3. Make up volume to 1 liter with distilled water. Reference: Nature protocols
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25 mM Tris-buffered saline (TBS) recipe |
| 1. Dissolve 8 g NaCl, 0.2 g KCl and 3 g Tris base in 800 ml distilled water. 2. Add 0.015g phenol red. 3. Adjust to pH 7.4 with HCl. 4. Make up volume to 1 liter with distilled water. 5. Sterilize by autoclaving. Reference: Nature protocols
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1M Tris-HCl, pH 6-8 recipe |
| 12.1g Tris base (MW 121.14) in 100ml H2O (adjust pH with concentrated HCl) Reference:
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2% X-gal recipe |
| Dissolve X-gal in dimethylformamide at a concentration of 20 mg/ml solution. Store at -20c and avoid light. Reference:
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1x YT recipe |
| tryptone 16 g yeast extract 10 g NaCl 5 g deionized H2O, to 900 ml Adjust the pH to 7.0 with 5 N NaOH. Adjust the volume of the solution to 1 liter with H2O. Sterilize by autoclaving. Reference:
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