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extract RNA from yeast by TRIzol
Posted by: karla (IP Hidden, New member, 3)
Date: April 18, 2005 10:44PM

Hi all:

I use Trizol to extract RNA from yeast. But it seems that I can not get any RNA but a lot of DNA
contamination.

Hope someone could help me.Wait for your answer.

Have a nice day!


Karla

 

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Re: extract RNA from yeast by TRIzol
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: April 22, 2005 10:37AM

I know of two reasons why this may happen with Trizol extraction. The first way to get DNA contamination is to homogenize the sample in too small a reagent volume- try adding more trizol to subsquent samples. Remember the sample volume should not exceed 10% of the volume of Trizol used for homogenization. The second is if the samples used for extraction contained organic solvents (ethanol, DMSO), strong buffers, or an alkaline solution. If this is the case you will need to 'clean up' your samples before proceeding with extraction. The protocol varies depending on what solvent is present in your samples.

 

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Re: extract RNA from yeast by TRIzol
Posted by: karla (IP Hidden, New member, 3)
Date: April 27, 2005 09:03AM

Thank you very much for your advice. I will have a try. Hope it works.
Have a wonderful time in this wonderful season!

 

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Re: extract RNA from yeast by TRIzol
Posted by: gedavidlin (IP Hidden, New member, 4)
Date: November 8, 2007 09:21PM

Dear kARLA


I just have the yeast extract RNA for you. If you have more interesting in that, please contact me by e-mail.


gedavidlin@yahoo.com



you are welcome to contact me.

 

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Re: extract RNA from yeast by TRIzol
Posted by: gedavidlin (IP Hidden, New member, 4)
Date: November 15, 2007 08:02PM

I ALSO HAVE YEAST EXTRACT RNA-Na, THE 5'-MONONUCLEOTIDES(AMP,CMP,UMP,GMP) PRODUCTS. HOPE WE COULD HAVE BUSINESS IN NEAR FUTURE.



GEDAVIDLIN@YAHOO.COM





Edited 1 times. Last edit at 11/15/07 08:04PM by gedavidlin.

 

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