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DNA sequencing troubleshooting
Posted by: user (IP Hidden, Unregistered user, )
Date: November 22, 2004 01:05PM
A detailed DNA sequencing data interpretation and troubleshooting guide.
Link: [www.genomex.com] Source:University of Hawaii sequencing core ------------------------------------------------------------------------ nice troubleshooting. 2002-05-28 20:39:23 Frank (frankd@yahoo.com) ------------------------------------------------------------------------ it is very usefull 2003-01-02 02:13:12 igelisen (igelisen@yahoo.com) ------------------------------------------------------------------------ i am doing nested PCR for SEN virus using primers for 5 utr region. after doing sequencing PCR for the same, and sequencing by automated sequencer, blast search is matching the sequence with TT virus instead of SEN virus. even the sequence of amplicon from positive control is matching with TT virus. kindly help me out. 2003-03-26 10:32:26 arunkundra (arunkundra@yahoo.com) ------------------------------------------------------------------------ hi, could you tell me how to submit my question? I am a new user. thank you very much! 2003-09-02 01:14:15 Mary (huxilian@163.net) ------------------------------------------------------------------------ hi, could you tell me how to submit my question? I am a new user. thank you very much! 2003-09-02 01:14:40 Mary (huxilian@163.net) ------------------------------------------------------------------------ hi, by the way,I think maybe you used TT virus as plate instead of SEN,but you don't know about it . or maybe this exited a contamination in your PCR sytem. 2003-09-02 01:22:32 Mary (huxilian@163.net) ------------------------------------------------------------------------ I am looking for the full sequence of the pBabe puro vector. Thanks you 2004-03-22 02:03:39 afula (afula@md.huji.ac.il) ------------------------------------------------------------------------ Can someone please tell me where I can find the full sequence of pBabe puro vector? Thanks 2004-04-05 09:06:14 prajput (prajput@cyllene.uwa.edu.au) ------------------------------------------------------------------------ dna 2004-05-04 11:23:19 hassan abdelleh (hassanabdelleh@yahoo.com) ------------------------------------------------------------------------ Great Work! Cialis 2004-05-08 01:48:45 Cialis (cialis@123.com) ------------------------------------------------------------------------ hi,I'm looking for the full sequence of the binary vector pGA643.Can someone please tell me how or where to find it? 2004-05-15 04:08:22 Finding (ss6360@sina.com) ------------------------------------------------------------------------ I have been seeing a lot of shoulder C peaks which are read as extra C's in our sequence, could you tell me what this is due to, or how to avoid it? 2004-08-04 18:11:37 Jess (jlewis@ozgene.com)
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