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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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DNA precipitation by ethanol.
Posted by: Shivani (IP Hidden, New member, 2)
Date: April 14, 2008 12:37PM
hello everyone! I have been isolating DNA from blood using high salt precipitation method by NaCl. From past few months, at final step of DNA precipitation by ethanol I get DNA strands only in few tubes and not in all. Although when I add ethanol i can see some colloidal part but it gets dissolved completely before actually giving the DNA strands.
Is there any method by which I can precipitate DNA from this solution??? or is there any alternative to this step? my samples are very crucial, can not afford to lose more...please suggest
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