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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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Direct PCR from blood
Posted by: kshipra (IP Hidden, New member, 1)
Date: March 17, 2008 09:00AM
Hey there, can someone help me with this. I want to detect bacteria in blood samples using PCR. But the problem is that I want to directly apply PCR on whole blood without DNA extraction. I have done PCR on plasma after its separation from whole blood to detect the bacteria but PCR is having a very low sensitivity with that.
Please, if any one has any suggestions to improve upon this please do respond.
Re: Direct PCR from blood
Posted by: elm3r (IP Hidden, New member, 3)
Date: April 11, 2008 08:49AM
there seems to be something in ScienceDirect:
[www.sciencedirect.com] if you don't have a access, reply to this post to say it.
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