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CO-IP question
Posted by: vladimir (IP Hidden, New member, 1)
Date: March 15, 2008 09:39PM
How crucial is the following step in CO-IP protocol.
Add Monoclonal Antibody, rock 4-6 hours 4C. Add Protein A beads 10ul rock 1hr. I accidentally added Ab together with beads, then realized my mistake, spun beads down, added more Ab, put to rock 2hrs, spun down again, added a little more Ab. Rock 3hrs. My monoclonal Ab is very specific. Will this fly? Thanks.
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