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CO-IP question
Posted by: vladimir (IP Hidden, New member, 1)
Date: March 15, 2008 10:39PM

How crucial is the following step in CO-IP protocol.

Add Monoclonal Antibody, rock 4-6 hours 4C.
Add Protein A beads 10ul rock 1hr.

I accidentally added Ab together with beads, then realized my mistake, spun beads down,
added more Ab, put to rock 2hrs, spun down again, added a little more Ab. Rock 3hrs.
My monoclonal Ab is very specific.

Will this fly?

Thanks.

 

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