molecular cell biology lab troubleshooting Our community forum has a new face. Please visit Biowww community to find out how you can

  • Post threads, debates, activities, blogs, Polls, freelance solutions ...
  • Refer our new web 2.0 community to a friend and get the chance to win a free laser pointer for scientific presentations.
    • Home /Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Cytometry /Bio Jobs /Books /Bioproduct /Blog /Methods /Buffer
    Login  :: Register  :: Search forums  :: Top Users  :: Reagent
    Search forums
    Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
    Goto Thread: Previous > Next
    Goto: Forum List > Message List > > Search > Log In /or Register new user
    CO-IP question
    Posted by: vladimir (IP Hidden, New member, 1)
    Date: March 15, 2008 09:39PM

    How crucial is the following step in CO-IP protocol.

    Add Monoclonal Antibody, rock 4-6 hours 4C.
    Add Protein A beads 10ul rock 1hr.

    I accidentally added Ab together with beads, then realized my mistake, spun beads down,
    added more Ab, put to rock 2hrs, spun down again, added a little more Ab. Rock 3hrs.
    My monoclonal Ab is very specific.

    Will this fly?

    Thanks.

     

    > >


    Goto: Forum List•Message List•Search•Log In
    We are moving ... Please post to our new community forums

    Terms of service | Privacy policy

    Copyright 2000-2005 Biowww.net, All rights reserved