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Cell lysis buffer for real time RT-PCR
Posted by: tr0lle (IP Hidden, New member, 2)
Date: February 20, 2008 07:39AM
Hey
Does any know of a cell lysis buffer that is compatible with downstream real time RT-PCR analysis? I.e. a buffer that lysis the cells and which can be used directly for reverse transcription so no RNA isolation is required. If anyone would be willing to share such a buffer recipe or has come across a relevant paper/reference I would very much appreciate it. I am aware that such a lysis buffer is commercially available from, forexample, Ambion (Cells-to-cDNA™ II Cell Lysis Buffer) but it costs a fortune.
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