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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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Cell passage number and RNAi efficacy
Posted by: Kaire76 (IP Hidden, New member, 2)
Date: July 27, 2006 02:20PM
Has anyone experienced a loss in RNAi reproducibility with increased cell number passages? I've observed in Drosophila S2 cells that after 40 or so passes RNAi experiments get sketchy.
Re: Cell passage number and RNAi efficacy
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: August 1, 2006 11:02AM
Excessive passage numbers can reduce the effiency of transfection. I think the exact number of passages before this occurs would depend on the cell line, culture system, RNAi system and reagents used, etc.
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