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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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Polymyxin B
Posted by: charan (IP Hidden, Junior member, 14)
Date: July 25, 2006 06:57AM
Hi all,
I am trying to treat E coli cells with PolymyxinB in order to permeabilize them. Does anybody have an idea about the kinetics that is how long should the cells be grown in polymyxin B in order to ' poke holes ' in them without damaging them? Or at which stage should it be added , Can I add PB to the cells as they are growing or should I let them grow upto the desired OD and then add finally treat them with PB?? Thanx in advance.
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