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what other tag can i use beside Flag and HA
Posted by: qualityny (IP Hidden, New member, 3)
Date: February 15, 2006 12:22PM
i am trying to express connexin protein in Hela Cells , I ve been unsucessful to detect flag fusion protein.I am thinking to use a diffenrent tag rather than keep trying to detect the flag.
due to my experimental design i can't use HA tag ,Do you know Any other alternative Tag that I can use. Thanks
Re: what other tag can i use beside Flag and HA
Posted by: username132 (IP Hidden, Junior member, 15)
Date: February 19, 2006 06:41AM
This forum seems pretty useless to me and if it's come down to me trying to anwer people's questions then we're in dire straits! The only other tag I know of is maltose binding protein, MBP. I don't know the full range of vectors its available in but I think pMal-C2 from 'New England Biolabs' has it. By now you've probably fallen off you seat laughing at my ridiculous suggestion, but if by some strange phenomenon, I've actually said something even slightly useful, let me know, 'cause that would be way cool!!
Edit: I've just discovered pMal-C2 is a bacterial expression vector - I guess that means its no use? :( Edited 1 times. Last edit at 02/19/06 11:17AM by username132.
Re: what other tag can i use beside Flag and HA
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: February 19, 2006 11:14AM
List of epitope tags:
AU1 & AU5 Epitope Tags Beta Galactosidase D-Gal Epitope Tags E & E2 Epitope Tags ECS Epitope Tags (DDDDK) GAL4 Epitope Tags glu-glu Epitope Tags GST Epitope Tags HA Epitope Tags His Epitope Tags HSV Epitope Tags KT3 Epitope Tags MBP Epitope Tags Myc Epitope Tags Protein A Epitope Tags S1 Epitope Tags T7 Epitope Tags V5 Epitope Tags VSV-G Epitope Tags
Re: what other tag can i use beside Flag and HA
Posted by: Basshead (IP Hidden, New member, 4)
Date: February 22, 2006 07:27AM
You could consider the myc tag. It's fine for detection by immunofluorescence but you can have problems with IP's allthough I've found it's behaviour in IPs very protein-dependent. Seems like the myc epitope can be masked in some proteins - can usually be solved if you IP in RIPA.
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