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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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RT-PCR
Posted by: Nastja (IP Hidden, New member, 1)
Date: December 8, 2005 03:22AM
Hi!!!
I am working with RT-PCR. For cDNA synthesis I used QuantiTect Reverse Transcription kit (Qiagen). I have nice results:) but I need to be absolutely sure:) that my probes are free from DNA contamination. How can I check it? Thank you very much!!!
Re: RT-PCR
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: December 8, 2005 10:16AM
Treat RNA sample with DNase-free RNase will get ride of DNA contamination. Use primer pair flanking two different exons will allow you to tell whether or not there is still DNA contamination in your cDNA template.
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