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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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REPRODUCIBILITY OF PCR
Posted by: dal (IP Hidden, New member, 2)
Date: October 13, 2005 03:52AM
i have trying to amplify a 1 Kb fragment of bla tem gene but i have problems with repruducibility of my PCR. FOR some samples, sometimes the reaction work great and sometimes i get no product. i work with the same DNA extract.
Re: REPRODUCIBILITY OF PCR
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: October 14, 2005 12:23PM
There are too many things you need to consider to troubleshoot. To do it systematically follow some troubleshooting instructions such as this one: [biologi.uio.no]
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