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DGGE troubles
Posted by: Ervija (IP Hidden, New member, 2)
Date: August 27, 2005 04:49PM

Hi:
I have two problems with the DGGE:
1.-Sometimes I can't obtain the equal fingerprint with the same sample under the same conditions. Do you have any recomendation?
2.-I have a lot of problems with the silver staining. I clean my glass material with nitric acid 50%, and I use a kit (biorad) but the strips are very weakly or the background is black when I can watch the stripes good.
Beforehand Thanks for your help

 

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