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RE: S2 cell responsiveness to insulin
Posted by: lutan (IP Hidden, New member, 0)
Date: June 12, 2002 11:24PM

> >I have trouble getting S2 Drosophila melanogaster
> stimulated >by insulin.I have tried a number of Western
> blots but they >did not show any significant difference
> compared to the >control. Does anyone know why it is that my
> cells are not >responding?Any possible suggestions would be
> greatly >appreciated.
>
> Drosophila S2 cells are not complete cells, that is, they do
> not possess all of the same proteins as whole Drosophila.
> Also, where did you get your S2 cells from? According to
> ATCC, they senesce after 40 passages. However, Imagene
> Schneider (founder of the cells) has cultured these cells at
> WRAIR for over 800 passages, makes me wonder about why they
> now senesce. Are you sure that S2 cells have an insulin
> receptor and the necessary signalling components?
>
If I am not wrong, S2 cells do have insulin receptor and signal transduction pathway.

 

> >


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