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RE:Mutagenesis - large insertion
Posted by: huangz123 (IP Hidden, Junior member, 19)
Date: June 11, 2002 01:15PM

> I have been trying to insert a large sequence (~100bp) using
> Stratagene Exsite mutagenesis kit and have not had any luck
> so far. Do any of subscribers have any experience making
> such large insertion? There is no convenient restriction
> sites or possibility to insert restriction sites. Any
> suggestions are appreciated. Thank you.
>
They have been able to insert 122 bp using this kit. You may want to contact their techline directly.

 

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