:: Search forums :: Top Users :: Reagent
Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
|
degenerate primers
Posted by: kumarsysu (IP Hidden, New member, 9)
Date: August 14, 2005 09:39PM
hi
i had been trying out the degenerate primers with cDNA libraries. I have few questions: 1) why do i get many bands instead of our expected fragment alone? 2) how to differentiate between the bands that are formed by primer dimers and the bands out of sheer complimentarity? 3) should the expected fragment be present at the exact bp lenght or can it be little more? thanks senthil kumar Kumar
|
We are moving ... Please post to our new community forums
