| |||||||||
| Login :: Register :: Search forums :: Top Users :: Reagent |
|
Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
|
Strange DNA contamination
Posted by: ak19l (IP Hidden, New member, 1)
Date: February 25, 2008 03:12AM
Hi,
I have had a mysterious DNA contamination again and again after plasmid transformation to DH5alpha E.coli cells. I have checked that the contamination isn't in ligation, transformation, DNA purification or restriction reagents, component cells, loading buffer or water. But from time to time strange ~2500 bp and ~4000 bp bands appear in gel electrophoresis after analytical RE digestion. The sizes are the same regardless of RE I use, brightness of the bands varies. Do you have any ideas what causes this and how can I get rid of it? Thanks!
|
We are moving ... Please post to our new community forums
|
|
||||||||