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MEF, 3T3, Swiss3T3, NIH3T3
Posted by: aniu1229 (IP Hidden, New member, 8)
Date: July 12, 2005 04:18AM

could anyone explain to me, what is the relation and difference among these several cell lines?
thanks a lot!

 

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Re: MEF, 3T3, Swiss3T3, NIH3T3
Posted by: aniu1229 (IP Hidden, New member, 8)
Date: July 12, 2005 04:18AM

and why NIH3T3 has to be cultured in CS contained medium, but not FCS?

 

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Re: MEF, 3T3, Swiss3T3, NIH3T3
Posted by: aniu1229 (IP Hidden, New member, 8)
Date: July 12, 2005 04:23AM

sorry, one more question.
I saw in the paper, when people did serum starvation, these starved for 3-4 days. but in my case, even after starvation in 0% FBS for 1 day, cells kept growing, is there anyone explain this?
Thanks a lot!

 

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Re: MEF, 3T3, Swiss3T3, NIH3T3
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: July 20, 2005 11:15AM

To answer the first question: All of the cell lines are mouse embryonic fibroblast (fibroblast-like). This is where the term MEF (murine embryonic fibroblast) comes from. NIH 3T3 was established from an NIH Swiss mouse embryo (Swiss3T3). As for how the cell lines are different I suggest you do a MEDLINE search of studies that used the cell lines for inhibition and activation studies. I am not certain what differences would be of the most importance to you.
To answer your second question: I am not sure what paper you are referring to, but other researchers have grown NIH 3T3 in FCS as well as CS. Fetal calf serum can contain some growth or differentiation factors that are not found in calf serum derived afterbirth. Perhaps the researchers in your paper were trying to avoid the addition of these.
To answer your third question: many cell lines can survive serum deprivation for short periods of time. if the paper noted that the cells perished after 1 day, you may want to check how you switched the cells over to 0% serum media. it is possible that some residual media containing serum was left behind.

 

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thanks a lot for the explanation!
Posted by: aniu1229 (IP Hidden, New member, 8)
Date: July 20, 2005 03:19PM

I will check more information

 

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