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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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Weird PCR result
Posted by: hanspet (IP Hidden, New member, 1)
Date: October 12, 2006 06:39PM
This PCR was made with primers that have been working really well before, just a few days ago, giving a single sharp band. But today, instead of a clean band, I got a band followed by a very strong smear... this smear was present in all the samples, including the negatives. My first guess is that the primers went bad.. but this is a new stock recently ordered. What else could it be? bad taq? bad pcr machine?
thanks! hans
Re: Weird PCR result
Posted by: fraz (IP Hidden, New member, 1)
Date: February 12, 2007 12:35AM
You have to track back in mind about the addition/ change of any reagent tubes (including dNTPs, Taq, water and rest. As having bands in negative sample means something goes wrong with ur reagents . This may be due to contamination, Easy trick is to prepare all reagents New working stocks.
best wishes Fraz
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