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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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Gel mobility shift assay: unusual band shape
Posted by: mroginskaya (IP Hidden, New member, 1)
Date: September 29, 2006 03:23PM
In my gel mobility shift assay, the bands corresponding to the DNA-protein bound form are unusually shaped; instead of being regularly shaped like a horseshoe, the complex tends to accumulate around the edges of the well. What might be the reason of this? The protein is known to be a fairly good DNA binder, with a Kd around 25 nM.
Thank you, Marina
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