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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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problems isolating BAC
Posted by: nac (IP Hidden, New member, 1)
Date: September 28, 2006 04:36PM
Dear all,
I have problems in isolating high amounts of bacterial artificial chromosomes (BAC`s). We just moved to a newe building leaving our ultra centrifuge behind since it was old, broken and not prepared anymore. Now I have to find a good protocol for doing miniprep using microfuges. The yield is ofcourse much lower but what can i do to increase this yield? What are the critical steps i have to take? And is it possible to seperate BAC`s from genomic E. coli DNA since it is moth circular DNA? Thanks a lot for any suggestions. Nac
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