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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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How long can I leave colonies on agar plates?
Posted by: foxy (IP Hidden, New member, 2)
Date: April 4, 2006 01:21AM
I plated out some bacteria containing my plasmid before the weekend, and have left them in the incubator for 4 days! Will they still be alright to use, or will they have lost the plasmid? Thanks
Re: How long can I leave colonies on agar plates?
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: April 5, 2006 01:26PM
better to discard the plate. clony might not be single clony anymore and antibiotics may lost activity already.
Re: How long can I leave colonies on agar plates?
Posted by: fatboym (IP Hidden, Junior member, 20)
Date: May 4, 2006 07:02AM
It is ok to store it at 4oC. Wrap it with parafilm and you may use it until a month later. For longer storage use glycerol stock
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