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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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ChIP
Posted by: Pak (IP Hidden, New member, 1)
Date: December 16, 2005 09:41AM
Hi Everyone,
I will be doing Chromatin Immunoprecipitation (ChIP) and have a question regarding the PCR. Can anyone tell me what does the final PCR bands indicates: a. Does they indicate the amount of IP'd protein on the promoter, or b. Does the PCR bands only indicate the amount of promoter (DNA) attached to my IP'd protein. I was pretty much convinced with answer b. but yesterday got into some confusion. Any help will be appreciated. Thank you very much in advance, Pak
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