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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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Total RNA per cell
Posted by: maultm (IP Hidden, New member, 1)
Date: November 1, 2005 03:37PM
I'm trying to isolate total RNA from Rat bone marrow for taqMan PCR. We've never isolated RNA from this type of cell before and don't know what to expect in terms of yield. Can anyone tell me from their experience how much RNA per cell I can expect to get? This will be helpful for determining how many wells of cells I need to isolate RNA from. Thanks!
Timothy Maul
Re: Total RNA per cell
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: December 7, 2005 09:21AM
The yield should be similar to RNA isolation from whole blood. Normally 1-5ml is enough for RNA isolation. Vendors like Roche and others have such kit to improve the yield and purity.
maultm Wrote: ------------------------------------------------------- > I'm trying to isolate total RNA from Rat bone > marrow for taqMan PCR. We've never isolated RNA > from this type of cell before and don't know what > to expect in terms of yield. Can anyone tell me > from their experience how much RNA per cell I can > expect to get? This will be helpful for > determining how many wells of cells I need to > isolate RNA from. Thanks! > > Timothy Maul
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