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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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pvex vector sequence
Posted by: colababy (IP Hidden, New member, 4)
Date: December 6, 2005 02:21PM
It seems that so many papers use pvex (plasmid vector with t7 promoter) or a derivative, but they never cite a source that would give the sequence to the plasmid. Are there any plasmid sequence databases? I need to know what restriction enzymes to use.
Re: pvex vector sequence
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: December 7, 2005 09:13AM
If you couldn't find the sequence and map from internet why don't just send request to paper authors? When submit their manuscript most journals require authors to have all materials and methods available for public. Good luck.
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