DNA concentration
Posted by: kishore (IP Hidden, New member, 3)
Date: November 5, 2005 01:49AM
I WANT TO THE FORMULAE TO CALCULATE DNA CONCENTRATION PRESENT IN THE SAMPLE VOLUME
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Re: DNA concentration
Posted by: saritamalik (IP Hidden, New member, 2)
Date: November 5, 2005 05:19AM
hi
here's the method of spectrophotometric detection of DNA
make dilution of DNA : 1:499
take O.D. in UV range 260
use the formula
1.0 OD = 50 ng of ds DNA/ml
hope this solves your problem
best wishes
and
regards
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