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    luciferase assay in HCV
    Posted by: ronala (IP Hidden, New member, 2)
    Date: October 13, 2005 08:44AM

    Hi,

    I'm currently working with the HCV replicon, trying a luciferase assay. It works pretty well, but when I compare my counts to the litterature, they're between 1-2 logs lower. Does anyone have a method for getting higher counts? Generally I work in 96W, though I did try 12W and then trypsinizing and lysing, but the results from that were so-so. On a similar note, has anyone heard of opaque welled plates in a format larger than 96W (ie 12, 24...) that could be used in a luminecence counter?

    Thanks a bunch.

     

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