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    Tranfection of Non adherent murine lymphocytescells
    Posted by: Paul wafula (IP Hidden, New member, 2)
    Date: July 18, 2005 06:20AM

    Hello,
    I have been attempting to transfect murine lymphocytes using viral particles expressing my gene of interest tagged to GFP. The lymphocytes are non adherent.
    I have attempted transfecting using polybrene but no success. Anyone has an idea on how to deal with this problem??
    Kind regards,
    paul

     

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