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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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dna purification
Posted by: fatboym (IP Hidden, Junior member, 20)
Date: May 23, 2005 04:49PM
i used a marligen kit to purify from agarose gel a 4700bp DNA fragment that was produced after ë phage digestion. After the purification I run on an agarose gel some of the product for confirmation and I had a single band at circa 4000bp. Any suggestions?
Re: dna purification
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: May 24, 2005 01:44PM
i dont know much about the marligen kit, but it sounds as if your product may becoming broken down. perhaps due to a lack of DNase inhibitors.
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