| |||||||||
| Login :: Register :: Search forums :: Top Users :: Reagent |
|
Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
|
Digested DNA purified from agarose gel
Posted by: lsjye (IP Hidden, New member, 1)
Date: April 11, 2005 09:52AM
Hi, I am trying to purify digested genomic DNA (only select size of 19-22kb) from agarose gel, but I lost alot during the purification process. I am using QIAEX II Gel Extraction Kit. The purified DNA concentration is very low although I tried to decrease the final elution volume. So, is it any method to increase its concentration? I need the concentration up to around 500ng-1ug. Please help me if you guys know the way. Thanks.
Re: Digested DNA purified from agarose gel
Posted by: hz123 (IP Hidden, Unregistered user, )
Date: April 11, 2005 01:24PM
Ideally you would expect an recovery yield of around 75% for your large fragments. Did you use the fresh electrophoresis buffer? It would be worth to try using fresh buffers with correct pH. The major factor affects the beads binding is the pH. Good luck.
|
We are moving ... Please post to our new community forums
|
|
||||||||