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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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problem with DNA digestion
Posted by: Supaporn (IP Hidden, Unregistered user, )
Date: April 9, 2005 08:22AM
I have some problem with the DNA digestion. I cut the DNA with HindIII restriction enzyme according to the reaction: 15 ug DNA, 100u enzyme, 1x buffer incubate at 37C for 16 hours. the result show the uncomplete cutting. Can anyone suggest me
Re: problem with DNA digestion
Posted by: john36 (IP Hidden, Unregistered user, )
Date: April 9, 2005 08:31AM
My suggestions:
1. double check the DNA concentration. If it is genomic DNA it should not too viscous to do the digestion. 2. double check the buffer compatibility. Use the digestion buffer chart and buffer from the same vendor unless you are sure the buffer compositions from different vendors are identical. 3. good luck.
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