| |||||||||
| Login :: Register :: Search forums :: Top Users :: Reagent |
|
Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
|
How to estimate the size of plasmid?
Posted by: antibiotic (IP Hidden, New member, 1)
Date: April 5, 2005 11:01AM
Hi,
I am trying to estimate the size of a plasmid by doing restriction digestion with a bunch of enzymes. But my problem now is, I cannot get purified plasmid from gel extraction (I used QiaQuick Gel Exaction kit, which is good for DNA fragement less than 10kb.). I used NanoDrop to measure the plasmid concentration, but it was very low and not pure. The plasmid matched 2.2kb of DNA ladder on agarose gel. But that was probably supercoiled. The size must be larger than that. Should I try QiaQuick II gel extraction kit (fragment up to 50kb)? I am also afraid that the plasmid will be cut during extraction by either kit. Can anyone help me out on this? Thanks a million! -Antibiotic
Re: How to estimate the size of plasmid?
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: April 5, 2005 03:58PM
Your plasmid matched 2.2kb DNA marker should be easy to purified. Sometime, Qiagen's kit gave unfavored performance with unknown reason (for example, low yield but high purity?????....) and the most annoying thing to me is they charge well too much. Since then I switch to GenCatch gel extraction kit without any trouble - higher yield with high purity but lower cost.
|
We are moving ... Please post to our new community forums
|
|
||||||||