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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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silver staining troubleshooting
Posted by: ana (IP Hidden, New member, 1)
Date: March 2, 2005 10:40AM
Can anyone please tell me why sometimes the silver staining of a SSCP gel gets inverted? What I mean is: the DNA bands stain white and the remaining is brown. This happens either with fresly made solutions or with already used solutions witch stained properly. Any hint is needed. Thank You Ana
Re: silver staining troubleshooting
Posted by: pkaneu (IP Hidden, Unregistered user, )
Date: March 8, 2005 10:07PM
Ana, It sounds like you loaded too much protein. Lower your loading as silver staining gives as low as few ng sensitivity.
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