| |||||||||
| Login :: Register :: Search forums :: Top Users :: Reagent |
|
Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
|
dephosphorylation chew up
Posted by: Jula (IP Hidden, New member, 4)
Date: January 19, 2005 10:59AM
Hello,
I am having a slight problem constructing a cosmid library. Whenever I try to do a dephosphorylation there seems to be hardly any DNA left at the end. I used 0.1U CIAP/20pmol DNA. Therefore I run out of vector pretty fast, too. Now I was wondering whether there are any tricks not to loose all you DNA or whether I can grow some more of my 7.9kb cosmid by transforming it into the XL1-Blue cells or do I have to do an electroporation because it is too big? Any suggestions are very welcome. Thanks.
Re: dephosphorylation chew up
Posted by: biowww (IP Hidden, Regular member, 31)
Date: January 19, 2005 12:39PM
I searched the troubleshooting database using keywords dephosphorylation or CIP and it returned 41 hits. Hope it is helpful to you. Click for the result -> [biowww.net]
[biowww.net] Jula Wrote: ------------------------------------------------------- > Hello, > > I am having a slight problem constructing a cosmid > library. Whenever I try to do a dephosphorylation > there seems to be hardly any DNA left at the end. > I used 0.1U CIAP/20pmol DNA.
|
We are moving ... Please post to our new community forums
|
|
||||||||