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RNA interference is ineffective as a routine method for gene silencing ...
Posted by: mwil (IP Hidden, New member, 7)
Date: June 26, 2005 08:56PM
Published articles - International Journal of Biological Sciences
[www.biolsci.org] ISSN 1449-2288 Hernández VH, Bueno D. RNA interference is ineffective as a routine method for gene silencing in chick embryos as monitored by fgf8 silencing. Int J Biol Sci 2005; 1:1-12. [www.biolsci.org]
Re: RNA interference is ineffective as a routine method for gene silencing ...
Posted by: jonmoulton (IP Hidden, Junior member, 15)
Date: June 28, 2005 08:18AM
Morpholino oligos offer an effective alternative.
Draper BW, Morcos PA, Kimmel CB. Inhibition of zebrafish fgf8 pre-mRNA splicing with morpholino oligos: A quantifiable method for gene knockdown. Genesis. 2001 Jul;30(3):154-6. Tucker RP. Using Antisense Morpholino Oligos to Knockdown Gene Expression in the Chicken Embryo. Acta Histochemica et Cytochemica, 2002, vol. 35, no. 5, pp. 361-366 Kos R, Tucker RP, Hall R, Duong TD, Erickson CA. Methods for introducing morpholinos into the chicken embryo. Dev Dyn. 2003 Mar;226(3):470-7. See the searchable online publication database at www.gene-tools.com for more examples. - Jon Jon D. Moulton, Ph.D. GENE TOOLS, LLC jmoulton@gene-tools.com
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