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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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Extra band at ¡À 120 kDa in sample buffer
Posted by: hgosker (IP Hidden, New member, 0)
Date: July 29, 2004 09:37AM
We experience something funny. We run normal SDS page gels. On our blots, we see an extra band (roughly estimated)at 120 kDa. It also appears in lanes in which we just loaded sample buffer (mercapto-eth, glycerol, bromophenol blue and Tris). Sample buffer was freshly made several times. Band appears using different secondary (peroxidase labeled) antibodies. Omitting primary antibody still yields the extra band. Anybody knows what's going on?
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