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    protein purification
    Posted by: user (IP Hidden, Unregistered user, )
    Date: July 5, 2004 03:21PM

    I have purified a C- terminal His tag protein of pI 6.93, The protein is clear but on storage at -20 or dialysis (50 mM Potassium phosphate buffer, pH 7.0) it gets precipitated. I appreciate suggestions to solve this problem.

    Mona

     

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