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Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
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solubility problem after purification of his-tag protein
Posted by: zhang (IP Hidden, New member, 0)
Date: June 14, 2004 04:52AM
I purified one his-tag protein around 80kDa from Baculo system under native condition. Short time after elution (with 200mM Imidazole), my protein began to precipitate. I tried to add glycerol or triton and change the salt concentration to solubilize the protein, but it did work. Could you give me some suggestions? Thank you very much.
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