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    RE:contamination
    Posted by: user (IP Hidden, Unregistered user, )
    Date: June 6, 2004 11:59AM

    before any troubleshooting I would change everything fresh and not use the old stock solutions in the new RT-PCR.

    > Only with one pair of primers after RT-PCR, I see
    > contamination (perfect ~20bp ladder on 8% PAGE) in some
    > samples (usualy in thoose with low templete concentration
    > and usualy in the spring, but once in December) or in "no
    > template control". If some can help me to explain this, I`ll
    > send a picture of terrible gels.
    >

     

    > >


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