:: Search forums  :: Top Users  :: Reagent

Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
Goto Thread: Previous > Next
Goto: Forum List > Message List > > Search > Log In /or Register new user
alternative splicing
Posted by: lee2245 (IP Hidden, Junior member, 12)
Date: June 22, 2005 02:26PM

I got BAC clone for my interesting gene and I want to isolate entire promoter and gene to generate transgenic mice. However, I don't want to express the gene.
The reason why I want to put that gene along with promoter is that it helps the promoter efficienty.
I am going to put GFP after promoter. If I make out of frame of the gene by adding one base pair of nucleotide right after GFP, is it possible that I can block the downstream of genes?
Since there is two exons and one intron and the protein is a precursor which can be two other proteins by alternative splicing, I wonder making out of frame is good enough to block any translation of the genes.

Need comments from experts.

Thanks,

 

> >


Goto: Forum List•Message List•Search•Log In
We are moving ... Please post to our new community forums