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    Oligo precipitation
    Posted by: agallego (IP Hidden, New member, 0)
    Date: July 11, 2002 03:20PM

    I wonder if anyone can help me undo a mistake. I mistakenly diluted modified oligos in 20uM Tris buffer down to 10uM instead of the concentrated 100uM stock I wanted. I'm afraid the increase in Tris will affect my assay(virus load detection via RT-PCR). Does anyone have a suggested protocol for concentrating oligo's by precipitation while eliminating the salts?

    best regards,

    Alesia

     

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