molecular cell biology lab troubleshooting Our community forum has a new face. Please visit Biowww community to find out how you can

  • Post threads, debates, activities, blogs, Polls, freelance solutions ...
  • Refer our new web 2.0 community to a friend and get the chance to win a free laser pointer for scientific presentations.
    • Home /Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Cytometry /Bio Jobs /Books /Bioproduct /Blog /Methods /Buffer
    Login  :: Register  :: Search forums  :: Top Users  :: Reagent
    Search forums
    Position: Discussion forums > Bioscience biotechniques discussion forums > Molecular biology troubleshooting forum
    Goto Thread: Previous > Next
    Goto: Forum List > Message List > > Search > Log In /or Register new user
    Oligo precipitation
    Posted by: agallego (IP Hidden, New member, 0)
    Date: July 11, 2002 03:19PM

    I wonder if anyone can help me undo a mistake. I mistakenly diluted modified oligos in 20uM Tris buffer down to 10uM instead of the concentrated 100uM stock I wanted. I'm afraid the increase in Tris will affect my assay(virus load detection via RT-PCR). Does anyone have a suggested protocol for concentrating oligo's by precipitation while eliminating the salts?

    best regards,

    Alesia

     

    > >


    Goto: Forum List•Message List•Search•Log In
    We are moving ... Please post to our new community forums

    Terms of service | Privacy policy

    Copyright 2000-2005 Biowww.net, All rights reserved