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    Yeast's RNA extraction
    Posted by: Laura Varela (IP Hidden, New member, 0)
    Date: July 5, 2002 01:41PM


    Hi,
    I am trying to extract RNA from yeast. In the extraction buffer I intend to use aurintricarboxylic acid (ATA) as a RNase inhibitor. The problem is that when I measure the absorbance at 260 and 280 nm it seems to interfere. Can anyone give me a method to "clean up" the RNA before I quantitate it?

    Thank you very much

     

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