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Zebrafish in situ hybridization
Posted by: aline (IP Hidden, New member, 1)
Date: April 20, 2005 05:56AM



Hi,

I perform RNA in situ hybridization on zebrafish embryos at 10 somites stage.

I use the Roche DIG AP-conjugated Antibody and NBT/BCIP. My problem occur in the blocking step.

I am using now the blocking reagent powder with MABT blocking solution but i have a lot of

background red color. I really need a protocol to resolve this problem ( protocol without using sheep or

goat .. serum, because i don't have accessibility to those products now).


THANK YOU FOR THE HELP


ALINE

 

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Re: Zebrafish in situ hybridization
Posted by: Biju Joseph (IP Hidden, Unregistered user, )
Date: April 22, 2005 10:28PM

THis site might of use for you.
[bama.ua.edu]

 

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