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    Zebrafish in situ hybridization
    Posted by: aline (IP Hidden, New member, 1)
    Date: April 20, 2005 04:56AM



    Hi,

    I perform RNA in situ hybridization on zebrafish embryos at 10 somites stage.

    I use the Roche DIG AP-conjugated Antibody and NBT/BCIP. My problem occur in the blocking step.

    I am using now the blocking reagent powder with MABT blocking solution but i have a lot of

    background red color. I really need a protocol to resolve this problem ( protocol without using sheep or

    goat .. serum, because i don't have accessibility to those products now).


    THANK YOU FOR THE HELP


    ALINE

     

    > >

    Re: Zebrafish in situ hybridization
    Posted by: Biju Joseph (IP Hidden, Unregistered user, )
    Date: April 22, 2005 09:28PM

    THis site might of use for you.
    [bama.ua.edu]

     

    > >


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