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Position: Discussion forums > Bioscience biotechniques discussion forums > Immunohistochemistry In Situ Hybridization forum
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specific enolase and immunohistochemistry
Posted by: FabianBernal (IP Hidden, New member, 1)
Date: February 10, 2006 12:05PM
Hello everybody.
I was interested in checking the pressence of granulocytes in my tissue, related to MMP-9 expression. As a fast assay I was suggested to stain using chloroacetate specific enolase, that should be specific for granulocytes and mast cells,, but not monocytes/macrophages. However I have found a high degree of granulocyte infiltration and that _all_ my positive MMP-9 cells are positive for esterase. Does anybody know if there is any incompatibility of doing immunohistochemistry (peroxidase-DAB precipitation system) followed by chloroacetate esterase staining? Thank you for the answer, anyway.
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