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dapi staining before secondary antibody
Posted by: fatboym (IP Hidden, Junior member, 20)
Date: August 19, 2005 03:48AM
By mistake I have stained with dapi before adding my secondary goat anti-mouse fluorescent conjugated abtibody. Can I added now, or may I remove Dapi somehow?
Re: dapi staining before secondary antibody
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: August 19, 2005 09:17AM
I guess you can just continue secondary antibody staining. DAPI is a strong DNA minor groove binder (not sure if it is covalent binding) and you may manage to quench it (?). But I am afraid it will ruin your experiments.
Re: dapi staining before secondary antibody
Posted by: CArapinis (IP Hidden, New member, 3)
Date: March 10, 2006 07:40AM
Re malaka, it didn;t ruin my experiment and in fact it was particular well.. thanks for the post
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