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Position: Discussion forums > Bioscience biotechniques discussion forums > Immunohistochemistry In Situ Hybridization forum
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formamide in buffer?
Posted by: hikke (IP Hidden, New member, 1)
Date: May 15, 2006 02:37AM
Dear all,
Can anyone tell me why the use of formamide in wash buffers is necessary? After hybridization in 50% formamide at 37 degrees, wouldn't it be fine to wash at 60 degrees in 2xSSC or .6 x SSC or wahtever is best for your favourite probe? I see instead that most literature describes washing in 50% formamide and therefore at lower temperaturs. Difference RNA-DNA probes?? thanks
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